Journal: PNAS Nexus

Article Title: Single-cell mapping of the glycocalyx in the adult human and mouse ocular surface

doi: 10.1093/pnasnexus/pgaf348

Figure Lengend Snippet: Interspecies differences in ocular surface glycocalyx composition. A) UMAP projection of mouse ocular surface cells grouped by gene expression (left); bar chart showing the number of cells per cluster (center); and violin plots displaying representative markers for each cell cluster (right). B) Sankey diagram illustrating the distribution of mouse corneal cell types across lectin-based clusters (left); bar chart showing the number of cells per lectin cluster (center); and UMAP projection of mouse cells grouped by lectin labeling (right). C) Dot plot showing lectin binding patterns across mouse lectin clusters. D) Cross-species comparison of lectin labeling across shared cell clusters. Radial plots depict normalized expression values for each lectin, with human data in yellow and mouse data in blue. E) Representative images of the human and mouse cornea stained with WGA (red), SNA I (green), and DAPI (blue). The dotted line marks the epithelial–stromal boundary. Scale bars, 50 µm. F) Representative image of mouse conjunctiva stained with PNA (red), ConA (green), and DAPI (blue). Magnified views (right) show the region outlined by the dotted box. Arrowheads indicate goblet cells. Scale bar, 50 µm.

Article Snippet: Unconjugated lectins—including AAL, Maclura pomifera agglutinin (MPA), Maackia amurensis agglutinin I (MAA I), Concanavalin A (ConA), Datura stramonium agglutinin (DSA), wheat germ agglutinin (WGA), peanut agglutinin (PNA), and Erythrina cristagalli agglutinin (ECA)—as well as biotinylated Sambucus nigra agglutinin I (SNA I), were obtained from Vector Laboratories (Newark, CA, USA).

Techniques: Gene Expression, Labeling, Binding Assay, Comparison, Expressing, Staining